быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-BTK-Y223 Rabbit mAb (AP1136)
- Описание
- Характеристики
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Overview
Product name Phospho-BTK-Y223 Rabbit mAb Catalog No. AP1136 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0307 Background
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms.Immunogen information
Immunogen A synthetic phosphorylated peptide around Y223 of human BTK (Q06187). Sequence ALYDY Gene ID Swiss Prot Synonyms AT; ATK; BPK; XLA; IMD1; AGMX1; IGHD3; PSCTK1 Calculated MW 76kDa Observed MW 77kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples Raji+pervanadate Cellular location Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein 
Western blot analysis of extracts of Raji cells, using Phospho-BTK-Y223 antibody (AP1136) at 1:500 dilution.Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic phosphorylated peptide around Y223 of human BTK (Q06187). Isotype IgG