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Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-CDC37-S13 Rabbit mAb (AP1141)
- Описание
- Характеристики
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Overview
Product name Phospho-CDC37-S13 Rabbit mAb Catalog No. AP1141 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1602 Background
The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.Immunogen information
Immunogen A synthetic phosphorylated peptide around S13 of human CDC37 (Q16543). Sequence EVSDD Gene ID Swiss Prot Synonyms P50CDC37 Calculated MW 44kDa Observed MW 50kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, NIH/3T3, C6 Cellular location Cytoplasm 
Western blot analysis of extracts of various cell lines, using Phospho-CDC37-S13 Rabbit mAb (AP1141) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of extracts of C6 cells, using Phospho-CDC37-S13 Rabbit mAb (AP1141) at 1:1000 dilution.C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around S13 of human CDC37 (Q16543). Isotype IgG