быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-BRD4-T204 Rabbit mAb (AP1276)
- Описание
- Характеристики
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Overview
Product name Phospho-BRD4-T204 Rabbit mAb Catalog No. AP1276 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC50960 Background
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.Immunogen information
Immunogen A synthetic phosphorylated peptide around T204 of human BRD4 (NP_490597.1). Sequence ASTPP Gene ID Swiss Prot Synonyms CAP; MCAP; HUNK1; HUNKI; FSHRG4 Calculated MW 152kDa Observed MW 200kDa Applications
Reactivity Human, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa+Calyculin A, C6+Calyculin A Cellular location Chromosome, Nucleus 
Western blot analysis of extracts of various cell lines, using (AP1276) at1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen A synthetic phosphorylated peptide around T204 of human BRD4 (NP_490597.1). Isotype IgG