быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-CDK1-Y15 Rabbit mAb (AP1350)
- Описание
- Характеристики
-
Overview
Product name Phospho-CDK1-Y15 Rabbit mAb Catalog No. AP1350 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC55443 Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen A synthetic phosphorylated peptide around Y15 of human CDK1(NP_001777.1). Sequence GTYGV Gene ID Swiss Prot Synonyms CDC2; CDC28A; P34CDC2 Calculated MW 34kDa Observed MW 34kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:1000 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa+Hydroxyurea Cellular location Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle 
Western blot analysis of various lysates, using Phospho-CDK1-Y15 antibody (AP1350) at1:2000 dilution.HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic phosphorylated peptide around Y15 of human CDK1(NP_001777.1). Isotype IgG