быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-CREB1-S129 Rabbit pAb (AP1437)
- Описание
- Характеристики
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Overview
Product name Phospho-CREB1-S129 Rabbit pAb Catalog No. AP1437 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.Immunogen information
Immunogen A synthetic phosphorylated peptide around S129 of human CREB1. Sequence ILSRR Gene ID Swiss Prot Synonyms CREB; CREB-1 Calculated MW 36KDa Observed MW 37kDa Applications
Reactivity Human, Rat Tested applications WB Recommended dilution - WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T+λ-PP, C6+λ-PP Cellular location Nucleus 
Western blot analysis of 293T, using Phospho-CREB1-S129 Rabbit pAb (AP1437) at 1:400 dilution.293T cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of C6, using Phospho-CREB1-S129 Rabbit pAb (AP1437) at 1:400 dilution.C6 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen A synthetic phosphorylated peptide around S129 of human CREB1. Isotype IgG