быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-Chk1-S280 Rabbit mAb (AP1444)
- Описание
- Характеристики
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Overview
Product name Phospho-Chk1-S280 Rabbit mAb Catalog No. AP1444 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC62579 Background
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.Immunogen information
Immunogen A synthetic phosphorylated peptide around S280 of human Chk1(NP_001107593.1). Sequence VTSGG Gene ID Swiss Prot Synonyms CHK1 Calculated MW 54kDa Observed MW 54kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T+CA Cellular location Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center 
Western blot analysis of 293T, using Phospho-Chk1-S280 Rabbit mAb (AP1444) at 1:2000 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic phosphorylated peptide around S280 of human Chk1(NP_001107593.1). Isotype IgG