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Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-c-Abl-Y412 Rabbit pAb (AP1060)
- Описание
- Характеристики
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Overview
Product name Phospho-c-Abl-Y412 Rabbit pAb Catalog No. AP1060 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons.Immunogen information
Immunogen A synthetic phosphorylated peptide around Y412 of human ABL1 (NP_005148.2). Sequence DTYTA Gene ID Swiss Prot Synonyms ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; BCR-ABL; bcr/abl Calculated MW 123kDa Observed MW 123kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples C6 Cellular location Cytoplasm, Lipid-anchor, Mitochondrion, Nucleus, Nucleus membrane, cytoskeleton 
Western blot analysis of extracts of C6 cells, using Phospho-c-Abl-Y412 pAb (AP1060) at 1:1000 dilution or ABL1 antibody (A0282).C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-c-Abl-Y412 antibody (AP1060) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human appendix using Phospho-c-Abl-Y412 antibody (AP1060) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver using Phospho-c-Abl-Y412 antibody (AP1060) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around Y412 of human ABL1 (NP_005148.2). Isotype IgG