быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-β-Catenin-Y333 Rabbit pAb (AP0337)
- Описание
- Характеристики
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Overview
Product name Phospho-β-Catenin-Y333 Rabbit pAb Catalog No. AP0337 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen A phospho specific peptide corresponding to residues surrounding Y333 of human beta Catenin Sequence Email for sequence Gene ID Swiss Prot Synonyms EVR7; CTNNB; MRD19; NEDSDV; armadillo Calculated MW 85kDa Observed MW 85kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293 Cellular location Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole 
Western blot analysis of extracts from 293 cells, using Phospho-β-Catenin-Y333 antibody (AP0337).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A phospho specific peptide corresponding to residues surrounding Y333 of human beta Catenin Isotype IgG