быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-Bcl-XL-S62 Rabbit pAb (AP0314)
- Описание
- Характеристики
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Overview
Product name Phospho-Bcl-XL-S62 Rabbit pAb Catalog No. AP0314 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator.Immunogen information
Immunogen A phospho specific peptide corresponding to residues surrounding S62 of human Bcl-XL Sequence Email for sequence Gene ID Swiss Prot Synonyms BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S Calculated MW 26kDa Observed MW 30kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa Cellular location Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Mitochondrion inner membrane, Mitochondrion matrix, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein, centrosome, cytoskeleton, cytosol, microtubule organizing center, secretory vesicle, synaptic vesicle membrane 
Western blot analysis of extracts from HeLa cells using Phospho-Bcl-XL-S62 antibody (AP0314).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A phospho specific peptide corresponding to residues surrounding S62 of human Bcl-XL Isotype IgG