быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-c-Jun-S73 Rabbit pAb (AP0047)
- Описание
- Характеристики
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Overview
Product name Phospho-c-Jun-S73 Rabbit pAb Catalog No. AP0047 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.Immunogen information
Immunogen A synthetic phosphorylated peptide around S73 of human c-Jun (NP_002219.1). Sequence LASPE Gene ID Swiss Prot Synonyms AP1; p39; AP-1; cJUN; c-Jun Calculated MW 36kDa Observed MW 45-48kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:100 - 1:500
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples C6 Cellular location Nucleus 
Western blot analysis of C6, using Phospho-c-Jun-S73 antibody (AP0047) at 1:400 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human breast cancer using Phospho-c-Jun-S73 Rabbit pAb (AP0047) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around S73 of human c-Jun (NP_002219.1). Isotype IgG