быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-c-Fos-T232 Rabbit pAb (AP0038)
- Описание
- Характеристики
-
Overview
Product name Phospho-c-Fos-T232 Rabbit pAb Catalog No. AP0038 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.Immunogen information
Immunogen A synthetic phosphorylated peptide around T232 of human c-Fos (NP_005243.1). Sequence VATPE Gene ID Swiss Prot Synonyms p55; AP-1; C-FOS Calculated MW 41kDa Observed MW 62kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples HepG2 Cellular location Cytoplasm, Endoplasmic reticulum, Nucleus, cytosol Customer validation WB(Chlorocebus aethiops, Gallus gallus)
WB (Mus musculus)
Western blot analysis of extracts of HepG2 cells, using Phospho-c-Fos-T232 antibody (AP0038) at 1:1000 dilution or c-Fos antibody (A17351).HepG2 cells were treated by 10% FBS for after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around T232 of human c-Fos (NP_005243.1). Isotype IgG