быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-AKT2-S474 Rabbit pAb (AP0005)
- Описание
- Характеристики
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Overview
Product name Phospho-AKT2-S474 Rabbit pAb Catalog No. AP0005 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.Immunogen information
Immunogen A synthetic phosphorylated peptide around S474 of human AKT2 (NP_001617.1). Sequence QFSYS Gene ID Swiss Prot Synonyms PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA Calculated MW 56kDa Observed MW 60kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293 Cellular location Cell membrane, Cytoplasm, Early endosome, Nucleus, Peripheral membrane protein Customer validation WB(Homo sapiens, Rattus norvegicus)
Western blot analysis of extracts of 293 cells, using Phospho-AKT2-S474 antibody (AP0005) 1:1000 dilution..
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around S474 of human AKT2 (NP_001617.1). Isotype IgG