быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-C/EBPB-T235 Rabbit pAb (AP1055)
- Описание
- Характеристики
-
Overview
Product name Phospho-C/EBPB-T235 Rabbit pAb Catalog No. AP1055 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions.Immunogen information
Immunogen A synthetic phosphorylated peptide around T235 of human C/EBPB (NP_005185.2). Sequence PGTPS Gene ID Swiss Prot Synonyms TCF5; IL6DBP; NF-IL6; C/EBP-beta Calculated MW 36kDa Observed MW 38kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples NIH/3T3 Cellular location Cytoplasm, Nucleus Customer validation WB(Sus scrofa, Homo sapiens)
Western blot analysis of extracts of NIH/3T3, using Phospho-C/EBPB-T235 antibody (AP1055) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.NIH/3T3 cells were treated by Serum-starvation overnight at 37℃.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen A synthetic phosphorylated peptide around T235 of human C/EBPB (NP_005185.2). Isotype IgG