![[One Step] BAP31 Antibody Kit (RK05626)](/upload/resize_cache/iblock/d04/340_340_140cd750bba9870f18aada2478b24840a/wg4sjh2z35evoz4h2n7sypdk0pbfgpey.jpg "[One Step] BAP31 Antibody Kit (RK05626)")
быстрая доставка ~4 недели
Размер
- 50 μL
[One Step] BAP31 Antibody Kit (RK05626)
- Описание
- Характеристики
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Overview
Product name [One Step] BAP31 Antibody Kit Catalog No. RK05626 Host species Purification method Isotype Background
Immunogen information
Immunogen Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW Applications
Reactivity Tested applications Recommended dilution Storage buffer Key application Positive samples Cellular location 
Western blot analysis of extracts of HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of extracts of various cell lines, using BAP31 antibody (A7056) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunofluorescence analysis of U2OS cells using BAP31 antibody (A7056). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg BAP31 antibody (A7056). Western blot was performed from the immunoprecipitate using BAP31 antibody (A7056) at a dilution of 1:1000.
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Antibody type Primary Antibodies