быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
ADCY10 Rabbit pAb (A18473)
- Описание
- Характеристики
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Overview
Product name ADCY10 Rabbit pAb Catalog No. A18473 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene belongs to a distinct class of adenylyl cyclases that is soluble and insensitive to G protein or forskolin regulation. Activity of this protein is regulated by bicarbonate. Variation at this gene has been observed in patients with absorptive hypercalciuria. Alternatively spliced transcript variants encoding different isoforms have been observed. There is a pseudogene of this gene on chromosome 6.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human ADCY10 (NP_060887.2). Sequence MNTPKEEFQDWPIVRIAAHLPDLIVYGHFSPERPFMDYFDGVLMFVDISGFTAMTEKFSSAMYMDRGAEQLVEILNYHISAIVEKVLIFGGDILKFAGDALLALWRVERKQLKNIITVVIKCSLEIHGLFETQEWEEGLDIRVKIGLAAGHISMLVFGDETHSHFLVIGQAVDDVRLAQNMAQMNDVILSPNCWQLCDRSMIEIESVPDQRAVKVNFLKPPPNFNFDEFFTKCTTFMHYYPSGEHKNLLRLACTLKPDPELEMSLQKYVMESILKQIDNKQLQGYLSELR Gene ID Swiss Prot Synonyms SAC; HCA2; SACI; Sacy; hsAC; HEL-S-7a Calculated MW 187kDa Observed MW 48kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HepG2, Mouse testis Cellular location apical plasma membrane, cytoplasm, cytoskeleton, cytosol, dendrite, extracellular region, growth cone, mitochondrion, nucleus, perinuclear region of cytoplasm 
Western blot analysis of extracts of various cell lines, using ADCY10 antibody (A18473) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human ADCY10 (NP_060887.2). Isotype IgG