быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
ADAMTS1 Rabbit pAb (A15356)
- Описание
- Характеристики
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Overview
Product name ADAMTS1 Rabbit pAb Catalog No. A15356 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 718-967 of human ADAMTS1 (NP_008919.3). Sequence GGNGSTCKKISGSVTSAKPGYHDIITIPTGATNIEVKQRNQRGSRNNGSFLAIKAADGTYILNGDYTLSTLEQDIMYKGVVLRYSGSSAALERIRSFSPLKEPLTIQVLTVGNALRPKIKYTYFVKKKKESFNAIPTFSAWVIEEWGECSKSCELGWQRRLVECRDINGQPASECAKEVKPASTRPCADHPCPQWQLGEWSSCSKTCGKGYKKRSLKCLSHDGGVLSHESCDPLKKPKHFIDFCTMAECS Gene ID Swiss Prot Synonyms C3-C5; METH1 Calculated MW 105kDa Observed MW 105kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Mouse kidney, Mouse brain, Mouse liver, Rat brain, Rat liver Cellular location Secreted, extracellular matrix, extracellular space Customer validation WB(Ovis aries)
IHC(Ovis aries)
IF(Ovis aries)
Western blot analysis of extracts of various cell lines, using ADAMTS1 antibody (A15356) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of 293F cells using ADAMTS1 Rabbit pAb (A15356) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using ADAMTS1 Rabbit pAb (A15356) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ADAMTS1 Rabbit pAb (A15356) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using ADAMTS1 Rabbit pAb (A15356) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 718-967 of human ADAMTS1 (NP_008919.3). Isotype IgG