быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
ACAA2 Rabbit pAb (A15778)
- Описание
- Характеристики
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Overview
Product name ACAA2 Rabbit pAb Catalog No. A15778 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ACAA2 (NP_006102.2). Sequence MALLRGVFVVAAKRTPFGAYGGLLKDFTATDLSEFAAKAALSAGKVSPETVDSVIMGNVLQSSSDAIYLARHVGLRVGIPKETPALTINRLCGSGFQSIVNGCQEICVKEAEVVLCGGTESMSQAPYCVRNVRFGTKLGSDIKLEDSLWVSLTDQHVQLPMAMTAENLAVKHKISREECDKYALQSQQRWKAANDAGYFNDEMAPIEVKTKKGKQTMQVDEHARPQTTLEQLQKLPPVFKKDGTVTAGNA Gene ID Swiss Prot Synonyms DSAEC Calculated MW 42kDa Observed MW 50kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples LO2, A-549, HeLa, Mouse heart, Mouse liver Cellular location Mitochondrion Customer validation WB(Mus musculus)
Western blot analysis of extracts of various cell lines, using ACAA2 antibody (A15778) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ACAA2 Rabbit pAb (A15778) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using ACAA2 antibody (A15778) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using ACAA2 antibody (A15778) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ACAA2 antibody (A15778) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ACAA2 (NP_006102.2). Isotype IgG