![[KD Validated] Peroxiredoxin 1/PAG Rabbit pAb (A22008)](/upload/resize_cache/iblock/523/340_340_140cd750bba9870f18aada2478b24840a/hhcd9jdc595oai3muahaxi18hkhdctos.jpg "[KD Validated] Peroxiredoxin 1/PAG Rabbit pAb (A22008)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] Peroxiredoxin 1/PAG Rabbit pAb (A22008)
- Описание
- Характеристики
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Overview
Product name [KD Validated] Peroxiredoxin 1/PAG Rabbit pAb Catalog No. A22008 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human Peroxiredoxin 1/PAG (NP_001189360.1). Sequence MSSGNAKIGHPAPNFKATAVMPDGQFKDISLSDYKGKYVVFFFYPLDFTFVCPTEIIAFSDRAEEFKKLNCQVIGASVDSHFCHLAWVNTPKKQGGLGPMNIPLVSDPKRTIAQDYGVLKADEGISFRGLFIIDDKGILRQITVNDLPVGRSVDETLRLVQAFQFTDKHGEVCPAGWKPGSDTIKPDVQKSKEYFSKQK Gene ID Swiss Prot Synonyms PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A Calculated MW 22kDa Observed MW 22kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:1000 - 1:5000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa, MCF7, RAW264.7, Mouse kidney, Rat liver Cellular location cytoplasm, cytosol, extracellular exosome, extracellular space, melanosome, nucleus 
Western blot analysis of various lysates, using Peroxiredoxin 1/PAG Rabbit pAb antibody (A22008) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of extracts from wild type(WT) and Peroxiredoxin 1/PAG Rabbit pAb knockdown (KD) HeLa cells, using Peroxiredoxin 1/PAG Rabbit pAb antibody (A22008) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunofluorescence analysis of HeLa cells using [KO Validated] Peroxiredoxin 1/PAG Rabbit pAb (A22008) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of MCF7 cells using [KO Validated] Peroxiredoxin 1/PAG Rabbit pAb (A22008) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Peroxiredoxin 1/PAG Rabbit pAb (A22008) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using [KO Validated] Peroxiredoxin 1/PAG Rabbit pAb (A22008) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human Peroxiredoxin 1/PAG (NP_001189360.1). Isotype IgG