![[KD Validated] Bcl-XL Rabbit pAb (A22241)](/upload/resize_cache/iblock/a0e/340_340_140cd750bba9870f18aada2478b24840a/dih4lkz3ygwug05c1x85naj0sy0r1yyp.jpg "[KD Validated] Bcl-XL Rabbit pAb (A22241)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] Bcl-XL Rabbit pAb (A22241)
- Описание
- Характеристики
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Overview
Product name [KD Validated] Bcl-XL Rabbit pAb Catalog No. A22241 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Bcl-XL (NP_612815.1). Sequence MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAY Gene ID Swiss Prot Synonyms BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S Calculated MW 26kDa Observed MW 30kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples A-549, HeLa, L929, C6, PC-12 Cellular location centrosome, cytoplasm, cytosol, endoplasmic reticulum, mitochondrial inner membrane, mitochondrial matrix, mitochondrial outer membrane, mitochondrion, nuclear membrane 
Western blot analysis of extracts from normal (control) and Bcl-XL knockdown (KD) A-549 cells, using Bcl-XL antibody (A22241) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot analysis of various lysates, using Bcl-XL antibody (A22241) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot analysis of L929, using Bcl-XL antibody (A22241) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Bcl-XL (NP_612815.1). Isotype IgG