![[KD Validated] RHOT1/MIRO1 Rabbit pAb (A22551)](/upload/resize_cache/iblock/022/340_340_140cd750bba9870f18aada2478b24840a/mu9bdiynk5fcu6x7md6nstz27ivqqh1u.jpg "[KD Validated] RHOT1/MIRO1 Rabbit pAb (A22551)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] RHOT1/MIRO1 Rabbit pAb (A22551)
- Описание
- Характеристики
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Overview
Product name [KD Validated] RHOT1/MIRO1 Rabbit pAb Catalog No. A22551 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Predicted to enable GTP binding activity and GTPase activity. Involved in cellular homeostasis; mitochondrial outer membrane permeabilization; and mitochondrion transport along microtubule. Is integral component of mitochondrial outer membrane.Immunogen information
Immunogen Recombinant protein of human RHOT1/MIRO1. Sequence Email for sequence Gene ID Swiss Prot Synonyms ARHT1; MIRO1; MIRO-1 Calculated MW 71kDa Observed MW 75kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:100 - 1:500
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T, K-562, Mouse brain, Mouse testis, Rat testis Cellular location mitochondrial outer membrane, mitochondrion 
Western blot analysis of various lysates, using RHOT1/MIRO1 antibody (A22551) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of Rat testis, using RHOT1/MIRO1 antibody (A22551) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of extracts from wild type(WT) and RHOT1/MIRO1 knockdown (KD) 293T(KD) cells, using RHOT1/MIRO1 antibody (A22551) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using [KD Validated] RHOT1/MIRO1 Rabbit pAb (A22551) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human RHOT1/MIRO1. Isotype IgG