быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
ABF1 Rabbit pAb (A22789)
- Описание
- Характеристики
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Overview
Product name ABF1 Rabbit pAb Catalog No. A22789 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Identified as a protein that binds to abscisic acid response elements. May mediate transcriptional regulation of ABA responses.Immunogen information
Immunogen Recombinant protein of Arabidopsis thaliana ABF1. Sequence Email for sequence Gene ID Swiss Prot Synonyms abscisic acid responsive element-binding factor 1; AtABF1; F14J22.7; F14J22_7 Calculated MW 39kDa Observed MW Applications
Reactivity Arabidopsis thaliana Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples Seedling, inflorescence, Before inflorescence Cellular location nucleoplasm 
Western blot analysis of various tissues from Arabidopsis thaliana, using ABF1 antibody (A22789) at 1:600 dilution.NIH/3T3 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Резуховидка Таля(Arabidopsis thaliana) Immunogen Recombinant protein of Arabidopsis thaliana ABF1. Isotype IgG