![[KD Validated] CLIC1 Rabbit pAb (A23677)](/upload/resize_cache/iblock/a85/340_340_140cd750bba9870f18aada2478b24840a/3dbiiwwov70iyu3ekdk2l2tgkj7vylmj.jpg "[KD Validated] CLIC1 Rabbit pAb (A23677)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] CLIC1 Rabbit pAb (A23677)
- Описание
- Характеристики
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Overview
Product name [KD Validated] CLIC1 Rabbit pAb Catalog No. A23677 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.Immunogen information
Immunogen Recombinant protein of human CLIC1. Sequence NVTTVDTKRRTETVQKLCPGGQLPFLLYGTEVHTDTNKIEEFLEAVLCPPRYPKLAALNPESNTAGLDIFAKFSAYIKNSNPALNDNLEKGLLKALKVLDNYLTSPLPEEVDETSAEDEGVSQRKFLDGNELTLADCNLLPKLHIVQVVCKKYRGFTIPEAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKALK Gene ID Swiss Prot Synonyms G6; CL1C1; NCC27; CLCNL1 Calculated MW 27kDa Observed MW Refer to figures Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IHC 1:50 - 1:200
- IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples Cellular location Cell membrane, Cytoplasm, Nucleus, Nucleus membrane, Single-pass membrane protein 
Western blot analysis of MCF7, using CLIC1 Rabbit pAb (A23677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from wild type(WT) and CLIC1 knockdown (KD) HeLa cells, using CLIC1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of K-562 cells using [KO Validated] CLIC1 Rabbit pAb (A23677) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of THP-1 cells using [KO Validated] CLIC1 Rabbit pAb (A23677) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of Hela cells using 3 μg [KO Validated] CLIC1 Rabbit pAb (A23677). Western blot was performed from the immunoprecipitate using [KO Validated] CLIC1 Rabbit pAb (A23677) at a dilition of 1:2000.
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Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human CLIC1. Isotype IgG