быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
4-HNE Rabbit pAb (A24456)
- Описание
- Характеристики
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Overview
Product name 4-HNE Rabbit pAb Catalog No. A24456 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Immunogen information
Immunogen A synthesized peptide derived from 4-HNE. Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW 42-350kDa Applications
Reactivity Human Tested applications WB IF/ICC Recommended dilution - WB 1:1000 - 1:5000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Hela+4-HNE Cellular location 
Western blot analysis of Hela, using 4-HNE Rabbit pAb (A24456) at 1:1000 dilution.Hela treated with 4-Hydroxynonenal (4-HNE).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
Immunofluorescence analysis of HepG2 cells using 4-HNE Rabbit pAb (A24456) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthesized peptide derived from 4-HNE. Isotype IgG