быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
ZO-1 Rabbit pAb (A0659)
- Описание
- Характеристики
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Overview
Product name ZO-1 Rabbit pAb Catalog No. A0659 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1446-1748 of human TJP1 (NP_003248.3). Sequence SLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF Gene ID Swiss Prot Synonyms ZO-1 Calculated MW 195kDa Observed MW 195kDa Applications
Reactivity Human, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Rat lung Cellular location Cell junction, Cell membrane, Cytoplasmic side, Peripheral membrane protein, gap junction, tight junction Customer validation (mouse sertoli cell, Mouse embryonal fibroblast cell)
WB(Homo sapiens, Mus musculus, Sus scrofa, Rattus norvegicus, Gallus gallus, Anatinae, mmu, Horabagrus nigricollaris)
IF(Rattus norvegicus, Mus musculus, Homo sapiens, Cyprinus carpio, Sus scrofa)
IF(Mus musculus, Homo sapiens)
IHC(grass carp, Mus musculus, Rattus norvegicus, Homo sapiens)
WB(Mus musculus, Rattus norvegicus)
ICH(Rattus norvegicus)
ELISA(Rattus norvegicus, Mus musculus, Homo sapiens)
IF/ICC(Sus scrofa)
RT-PCR(Mus musculus)
IHC(Horabagrus nigricollaris, Mus musculus)
Western blot analysis of Rat lung, using ZO-1 Rabbit pAb (A0659) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 45s.
Immunofluorescence analysis of MCF7 cells using ZO-1 Rabbit pAb (A0659) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1446-1748 of human TJP1 (NP_003248.3). Isotype IgG