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Размер
- 100 μL
- 200 μL
- 50 μL
SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)
- Описание
- Характеристики
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Overview
Product name SARS-CoV-2 Nucleoprotein Rabbit pAb Catalog No. A18797 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-420 of coronavirus Nucleoprotein (P0DTC9). Sequence MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA Gene ID Swiss Prot Synonyms Calculated MW Observed MW 50kDa Applications
Reactivity SARS-CoV-2 Tested applications WB IF/ICC IP DB Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
- DB 1:500 - 1:2000
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples 293T-N, SARS-CoV Nucleoprotein NP protein（His-Tag） Cellular location Customer validation IF(Homo sapiens)
Co-IP(Homo sapiens)
WB(Homo sapiens)
Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein, using SARS-CoV-2 Nucleoprotein antibody (A18797) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of Recombinant Nucleoprotein of Coronavirus SARS-CoV-2，using SARS-CoV-2 Nucleoprotein antibody (A18797) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immobilized Recombinant SARS-COV-2 Nucleocapsid (RP01264) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Nucleoprotein Antibody (A18797) with a linear range of 3.12-200ng/mL.
Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SARS-CoV-2 Nucleoprotein antibody (A18797). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Nucleoprotein antibody (A18797) at a dilution of 1:1000.
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SARS-CoV-2 Nucleoprotein antibody (A18797). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Nucleoprotein antibody (A18797) at a dilution of 1:3000.
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Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity SARS-CoV-2 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-420 of coronavirus Nucleoprotein (P0DTC9). Isotype IgG