быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
SARS-CoV-2 N Protein Mouse mAb (A20142)
- Описание
- Характеристики
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Overview
Product name SARS-CoV-2 N Protein Mouse mAb Catalog No. A20142 Host species Mouse Purification method Affinity purification Isotype IgG1, kappa CloneNo. AMC0368 Background
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host SMAD3.Immunogen information
Immunogen Recombinant fusion protein of SARS-CoV-2 Nucleoprotein. Sequence Email for sequence Gene ID Swiss Prot Synonyms Nucleoprotein; NP Calculated MW 46kDa Observed MW 50kDa Applications
Reactivity SARS-CoV-2 Tested applications WB IF/ICC ELISA Recommended dilution - WB 1:500 - 1:1000
- ELISA 1:30000 - 1:120000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T Cellular location Customer validation WB(Mus musculus, Homo sapiens)
IP(Mus musculus)
ELISA(Mustela putorius furo)
Western blot analysis of extracts of normal 293T cells 293T transfected with N Protein, using SARS-COV-2 N Protein antibody (A20142) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immobilized Recombinant 2019-nCoV Nucleocapsid Protein (RP01264LQ) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Nucleoprotein Rabbit mAb (A20142) with a linear range of 3.12-200ng/mL.
Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 N Protein Mouse mAb (A20142) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Мышь Antibody type Primary Antibodies Reactivity SARS-CoV-2 Immunogen Recombinant fusion protein of SARS-CoV-2 Nucleoprotein. Isotype IgG1, kappa