![[KD Validated] METTL14 Rabbit mAb (A24396)](/upload/resize_cache/iblock/d26/340_340_140cd750bba9870f18aada2478b24840a/khzqiom4r7i2zslgq5kr2fnu87y52mv5.jpg "[KD Validated] METTL14 Rabbit mAb (A24396)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] METTL14 Rabbit mAb (A24396)
- Описание
- Характеристики
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Overview
Product name [KD Validated] METTL14 Rabbit mAb Catalog No. A24396 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59147 Background
Enables mRNA binding activity. Contributes to mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity. Involved in mRNA metabolic process; negative regulation of hematopoietic progenitor cell differentiation; and positive regulation of translation. Located in nucleoplasm. Part of RNA N6-methyladenosine methyltransferase complex.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human METTL14 (NP_066012.1). Sequence MDSRLQEIRERQKLRRQLLAQQLGAESADSIGAVLNSKDEQREIAETRETCRASYDTSAPNAKRKYLDEGETDEDKMEEYKDELEMQQDEENLPYEEEIYKDSSTFLKGTQSLNPHNDYCQHFVDTGHRPQNFIRDVGLADRFEEYPKLRELIRLKDELIAKSNTPPMYLQADIEAFDIRELTPKFDVILLEPPLEEYYRETGITANEKC Gene ID Swiss Prot Synonyms hMETTL14 Calculated MW 52kDa Observed MW 65KDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IP Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:1000 - 1:5000
- IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunoprecipitation Positive samples Raji, PC-3, A549 Cellular location Nucleus 
Western blot analysis of various lysates, using METTL14 Rabbit mAb (A24396) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
Western blot analysis of various lysates, using METTL14 Rabbit mAb (A24396) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] METTL14 Rabbit mAb (A24396) at dilution of 1:2000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using [KO Validated] METTL14 Rabbit mAb (A24396) at dilution of 1:2000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] METTL14 Rabbit mAb (A24396) at dilution of 1:2000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat testis using [KO Validated] METTL14 Rabbit mAb (A24396) at dilution of 1:2000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 300ug extracts of Raji cells using 3ug METTL14 Rabbit mAb (A24396). Western blot was performed from the immunoprecipitate using METTL14 Rabbit mAb (A24396) at a dilition of 1:500.
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Key applications Western blotting, Immunohistochemistry, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human METTL14 (NP_066012.1). Isotype IgG