быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
γ-Tubulin Rabbit mAb (A9657)
- Описание
- Характеристики
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Overview
Product name γ-Tubulin Rabbit mAb Catalog No. A9657 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1684 Background
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 231-451 of human γ-Tubulin (P23258). Sequence LVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDEMDTSREIVQQLIDEYHAATRPDYISWGTQEQ Gene ID Swiss Prot Synonyms TUBG; GCP-1; CDCBM4; TUBGCP1 Calculated MW 51kDa Observed MW 49kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples U-2 OS, MCF7, Jurkat, NIH/3T3, Mouse brain, Rat testis, Rat brain Cellular location Cytoplasm, centrosome, cytoskeleton, microtubule organizing center Customer validation IF(Homo sapiens)
Western blot analysis of extracts of various cell lines, using γ-Tubulin Rabbit mAb (A9657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded rat testis using γ-Tubulin Rabbit mAb (A9657) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using γ-Tubulin Rabbit mAb (A9657) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using γ-Tubulin Rabbit mAb (A9657) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Confocal imaging of NIH-3T3 cells using γ-Tubulin Rabbit mAb (A9657, dilution 1:100) (Red). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb (AC039, dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
Confocal imaging of U-2OS cells using γ-Tubulin Rabbit mAb (A9657, dilution 1:100) (Green). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb (AC039, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 231-451 of human γ-Tubulin (P23258). Isotype IgG