быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
α-Tubulin Mouse mAb (AC012)
- Описание
- Характеристики
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Overview
Product name α-Tubulin Mouse mAb Catalog No. AC012 Host species Mouse Purification method Affinity purification Isotype IgG1 CloneNo. AMC0479 Background
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant protein of human α-Tubulin Sequence Email for sequence Gene ID Swiss Prot Synonyms ALS22; TUBA1; H2-ALPHA Calculated MW 50kDa Observed MW 50kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:5000 - 1:10000
- IHC-P 1:50 - 1:200
- IF/ICC 1:100 - 1:200
- IP 1:100 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples U-87MG, 293T, HeLa, Jurkat, Mouse spleen, Mouse thymus, Mouse brain, Rat brain, Rat kidney Cellular location Cytoplasm, cytoskeleton Customer validation (HeLa treated by Starvatio and IGF)
WB(Rattus norvegicus, Homo sapiens, Mus musculus, Tegillarca granosa, Danio rerio, Artemisia scoparia, Sus domesticus)
IP(Homo sapiens)
IF(Mus musculus, Homo sapiens)
IP(Homo sapiens)
WB (Homo sapiens)
Western blot analysis of extracts of various cell lines, using α-Tubulin antibody (AC012) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded mouse brain using α-Tubulin Mouse mAb (AC012) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using α-Tubulin Mouse mAb (AC012) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Confocal imaging of HeLa cells using α-Tubulin Mouse mAb (AC012, dilution 1:200)(Green). The cells were counterstained with USO1 Rabbit mAb (A20950, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Мышь Antibody type Tag and Loading Control Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human α-Tubulin Isotype IgG1