быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
β-Tubulin Mouse mAb (AC021)
- Описание
- Характеристики
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Overview
Product name β-Tubulin Mouse mAb Catalog No. AC021 Host species Mouse Purification method Affinity purification Isotype IgG1 CloneNo. AMC0498 Background
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.Immunogen information
Immunogen Recombinant protein of human β-Tubulin Sequence Email for sequence Gene ID Swiss Prot Synonyms M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56 Calculated MW 50kDa Observed MW 50kDa Applications
Reactivity Human, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:5000 - 1:10000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
- IP 1:50- 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples SH-SY5Y, MCF-7, HepG2, HeLa, Mouse brain, Mouse lung, Rat brain Cellular location Cytoplasm, cytoskeleton Customer validation WB(Mus musculus, Homo sapiens, Rattus norvegicus, Arabidopsis thaliana, Crassostrea gigas, Mesocricetus auratus, Danio rerio)
IP(Mus musculus)
IF(Mus musculus)
IF(Mus musculus,Homo sapiens)
ICH(Mus musculus)
WB(Mus musculus)
Co-IP(Homo sapiens)
Western blot analysis of extracts of various cell lines, using β-Tubulin antibody (AC021) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded rat ovary using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat stomach using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Мышь Antibody type Tag and Loading Control Antibodies Reactivity Человек, Мышь, Крыса, Хомяк, Обезьяна, Коза, Хламидомонада Рейнгардта Immunogen Recombinant protein of human β-Tubulin Isotype IgG1