быстрая доставка ~4 недели
Размер
- 100 μL
Goat Anti-Mouse Anti-Rabbit IgG H(HRP polymer) (AS080)
- Описание
- Характеристики
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Overview
Product name Goat Anti-Mouse Anti-Rabbit IgG H(HRP polymer) Catalog No. AS080 Host species Goat Purification method Affinity purification Isotype IgG Background
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within both Mouse IgG and Rabbbit IgG. Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW 55kDa Applications
Reactivity Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples Mouse IgG, Rabbit IgG Cellular location 
Western blot analysis of extracts of various cell lines, using Goat Anti-Mouse Anti-Rabbit IgG H antibody (AS080) at 1:1000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Коза Antibody type Secondary Antibodies Immunogen A synthetic peptide corresponding to a sequence within both Mouse IgG and Rabbbit IgG. Isotype IgG







