Артикул
GB15938
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Recombinant Anti- FLAG Tag Mouse mAb
- Описание
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Product Information
Description
Flag Tag ( DYKDDDDK Tag ) mouse monoclonal antibody
Synonyms
DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG
Immunogen
Synthetic peptide : DYKDDDDK
Isotype
IgG
Purity
Affinity purification
Applications
Applications
Dilution
Positive Tissue
WB
1: 2000-1: 5000
Flag-Tag fusion protein
Background
The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Images
Western blot analysis of FLAG Tag (GB15938) at dilution of 1: 5000 Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.