Product Information
Description | alpha smooth muscle Actin rabbit polyclonal |
Protein full name | Actin, aortic smooth muscle |
Synonyms | Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS, GIG46,SMA, a-sma,sma |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouseα smooth muscle actin |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Cytoplasm |
Predicted MW. | 42 kDa |
Observed MW. | 42 kDa |
Uniprot ID | P62736,P62737, P62738 |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | heart, liver |
IHC | Human, Mouse, Rat | 1: 500-1: 1000 | lung cancer, lung, heart, liver |
IF | Human, Mouse, Rat | 1: 100-1: 300 | epityphlon, breast cancer, lung, stomach, brain, heart |
Background
Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (α-SMA, SMactin, alpha-SM-actin, ASMA). Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.Alpha-smooth muscle actin (α-SMA) is commonly used as a marker of myofibroblast formation.
Images
| Western blot analysis of alpha smooth muscle Actin (GB111364) at dilution of 1: 1000 Lane 1: HEK293 cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: Raw264.7 cell lysate Lane 6: Mouse heart tissue lysate Lane 7: Mouse liver tissue lysate Lane 8: Rat heart tissue lysate Lane 9: Rat liver tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded human lung cancer using alpha smooth muscle Actin (GB111364) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse lung using alpha smooth muscle Actin (GB111364) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse heart using alpha smooth muscle Actin (GB111364) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human epityphlon using alpha smooth muscle Actin (GB111364) at dilution of 1: 300 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse lung using alpha smooth muscle Actin (GB111364) at dilution of 1:1: 300 |
| Immunofluorescent analysis of paraformaldehyde-fixed Rat brain using alpha smooth muscle Actin (GB111364) at dilution of 1: 300 |
| Immunohistochemistry analysis of paraffin-embedded rat liver using alpha smooth muscle Actin (GB111364) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human breast cancer using alpha smooth muscle Actin (GB111364) at dilution of 1: 300 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse stomach using alpha smooth muscle Actin (GB111364) at dilution of 1: 300 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat heart using alpha smooth muscle Actin (GB111364) at dilution of 1: 300 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
