Product Information
Description | AKT1 rabbit polyclonal |
Protein full name | RAC-alpha serine/threonine-protein kinase |
Synonyms | AKT1 kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha,Akt1, Thymoma viral proto-oncogene, Akt |
Immunogen | KLH conjugated Synthetic peptide corresponding to Rat AKT1 |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. | 56 kDa |
Observed MW. | 60 kDa |
Uniprot ID |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1500 | heart, brain |
Background
The AKT1 protein plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4.This protein is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Images
| Western blot analysis of AKT1 (GB111114) at dilution of 1: 1000 Lane 1: NIH3T3 cell lysate Lane 2: PC12 cell lysate Lane 3: Mouse heart tissue lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat heart tissue lysate Lane 6: Rat brain tissue lysate |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
