Артикул
GB11938
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Anti - Flag Tag ( DYKDDDDK Tag ) Rabbit pAb
- Описание
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Product Information
Description
Flag Tag ( DYKDDDDK Tag ) rabbit polyclonal antibody
Synonyms
DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG
Immunogen
KLH conjugated Synthetic peptide corresponding to DYKDDDDK
Isotype
IgG
Purity
Affinity purification
Applications
Applications
Species
Dilution
Positive Sample
WB
tag
1: 500-1: 1000
Ttransfected 293T cells
Background
The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Images
Western blot analysis of Flag-Tag (GB11938) at dilution of 1: 500
Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.