Артикул
GB111251
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Anti -6 X His Tag Rabbit pAb
- Описание
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Product Information
Description
6 X His Tag rabbit polyclonal antibody
Synonyms
6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED
Immunogen
KLH conjugated Synthetic peptide corresponding to HHHHHH (6* His)
Isotype
IgG
Purity
Affinity purification
Applications
Applications
Species
Dilution
Positive Sample
WB
Tag
1: 500-1: 1000
His-Tag fusion protein
Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Images
Western blot analysis of His-Tag (GB111251) at dilution of 1: 500
Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.