ProductContent:

ProductDescription:

DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12, and it combines DMEM’s high concentrations of glucose, amino acids, and vitamins with F-12’s wide variety of components. DMEM/F-12 is widely used for supporting the growth of many different mammalian cells, including MDCK, glial cells, fibroblasts, human endothelia cells, and rat fibroblasts.

DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO₂environment to maintain physiological pH.

All raw material components used for Servicebio cell culture media products are screened through using strict quality control testing. Servicebio cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media are subjected to sterility test, pH, osmolality, endotoxin and cell culture test.

The major character of this DMEM is as follows:

l With 17.5 mM Glucose, 2.5 mM L-glutamine, 0.5 mM sodium pyruvate, phenol red.

l Without HEPES.

The complete formulation is available.

Storage:

Storage conditions: 2℃ to 8℃, protect from light

Shipping conditions: Ambient

Shelf life: 12 months from date of manufacture

Description:
DMEM/F-12 medium is a 1:1 mixture of DMEM and Ham 'F-12 medium.The improved DMEM/F-12 culture medium is more abundant in nutrients and contains more trace elements. And This product contains amino acids, vitamins, inorganic salts and other components needed for multi-type cell culture, without protein or growth factor.

Application:
DMEM/F-12 is suitable for the culture of mammalian cells and the culture of clonal density under low serum content. DMEM/F-12 is also used as a base medium for the development of serum-free media.

Storagecondition:
Storedat2-8℃,validfor12months.

Notes:
1. This product is filtered and sterilized by 0.1μmfilter membrane. Aseptic contamination shall be avoided during usage.
2. In order to maintain the best effect of usage, avoiding freeze and thaw.
3. This product is only used for lab research, not for diagnosis and treatment.