DMEM/High Glucose, with L-glutamine

Product introduction

DMEM/High Glucose, namely DMEM (Dulbecco's Modified Eagle Medium) high glucose medium, is improved on the basis of MEM medium and has been widely used in the culture of various cells. DMEM high-glucose medium is commonly used for fast-growing, low-adherence cells. It can be used for the culture of a variety of mammalian primary cells, such as primary fibroblasts, neural cells, glial cells, HUVEC, smooth muscle cells, etc., and can also be used for the culture of some cell lines, such as Hela, 293, Cos- 7 and PC-12, etc.

This product contains amino acids, vitamins, inorganic salts and other components required for various types of cell culture. It does not contain protein or growth factors. It needs to add 5-10% serum or serum-free supplements according to the cell type.

This product is filter sterilized through a 0.1 μm filter, pH 7.0-7.4, contains 25 mM D-glucose, adds 4 mM L-glutamine, contains phenol red indicator, does not contain sodium pyruvate, and does not contain HEPES buffer system.

storage and transportation

Transport at room temperature; store at 2-8°C, valid for 12 months.

Precautions

1. This product has been sterilized by filtration. When using, attention should be paid to aseptic operation to avoid contamination.

2. To maintain the best use effect, do not freeze and thaw.