Ham's F-12K Nutrient Medium Standard Sterile Cell Culture Medium

Productcontent

Productdescription

Ham’s F-12K (Kaighn’s) medium is a modification of Ham’s F-12 medium. Ham’s F-12K contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc. Compared to Ham’s F-12 medium, Ham’s F-12K cintains higher levels of all amino acids and sodium pyruvate. Ham’s F-12K medium was developed for primary hepatocytes, as well as for some rat and chicken liver cells in a reduced serum condition.

Ham’s F-12K contains no proteins or growth factors. Therefore, Ham’s F-12K requires supplementation, commonly with 10% Fetal Bovine Serum (FBS) according to specific cell type. Ham’s F-12K uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO₂environment to maintain physiological pH.

All raw material components used for Servicebio cell culture media products are screened through using strict quality control testing. Servicebio cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media are subjected to sterility test, pH, osmolality, endotoxin and cell culture test.

The major character of this Ham’s F-12K is as follows:

l With 7.0 mM Glucose, 2.0 mM L-glutamine, 2.0 mM sodium pyruvate, phenol red.

l Without HEPES.

The complete formulation is available.

Storage

Storage conditions: 2℃ to 8℃, protect from light

Shipping conditions: Ambient

Shelf life: 12 months from date of manufacture