Productcontent

Productdescription

Dr. Thomas McCoy originally formulated McCoy’s 5A medium as a modification of Basal Medium 5A. Different from other media, McCoy’s 5A contains the reducing glutathione, bacto-peptone, a high level of glucose, and also includes Hanks’s salts to enable use outside a CO₂incubator. McCoy’s 5A medium modified generally supports the propagation of many types of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung rat embryos and other tissues. It can also be used for growth of established cell lines and explants from biopsy tissues.

McCoy’s 5A medium modified contains no proteins, lipids, or growth factors. Therefore, McCoy’s 5A medium modified requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy’s 5A medium modified uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂environment to maintain physiological pH.

All raw material components used for Servicebio cell culture media products are screened through using strict quality control testing. Servicebio cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media are subjected to sterility test, pH, osmolality, endotoxin and cell culture test.

The major character of this McCoy’s 5A medium modified is as follows:

l With 16.67 mM Glucose, 1.5 mM L-glutamine, phenol red.

l Without sodium pyruvate and HEPES.

The complete formulation is available.

Storage

Storage conditions: 2℃ to 8℃, protect from light

Shipping conditions: Ambient

Shelf life: 12 months from date of manufacture