быстрая доставка ~4 недели
Размер
- 10000 U
- 2000 U
Taq DNA Ligase (RK20502)
- Описание
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Description
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands. The target strands need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37°C–75°C).
It is applicable to Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction, as well as Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification.Product Components
Components Cat. No. Taq DNA Ligase (40, 000 U/ml) RM20506 10X Taq DNA Ligase Reaction Buffer RM20131 Product Information
Feature Specification Product Source Purified from an E.coli strain containing the cloned ligase gene from Thermus thermophilus HB Applications Cloning Ligation, Seamless Assembly Unit Definition (Cohesive End Unit) One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1 µg of BstEII-digested λ DNA in a total reaction volume of 50 µl in 15 minutes at 45°C Storage Conditions 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C Storage Temperature -80°C Reaction Buffer or Mix 1X Taq DNA Ligase Reaction Buffer:
20 mM Tris-HCl, 25 mM Potassium Acetate, 10 mM Magnesium Acetate, 1 mM NAD, 10 mM DTT, 0.1% Triton X-100, pH 7.6@25°CHeat Inactivation No