Краситель SerRed для окрашивания нуклеиновых кислот, 10000×, водорастворимый (SerRed nucleic acid stain, 10000×, water-dissolvent), 100 мкл

Product information

Description/Introduction

SerRed is a sensitive, stable and relatively safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. The sensitivity of SerRed is much higher than EB, and destaining is not required. SerRed and EB have virtually the same spectral properties.

Storage and Handling Conditions

Storage at room temperature protected from light for two years，Shipping at room temperature.

Component

StainingProtocols

1.Precast agarose gel stain (the same as EB staining, recommended)

Add SerRed to molten agarose to make 1×final concentration (e.g. add 5 μL of SerRed 10,000 reagent to 50 ml of agarose).

Due to the high thermal stability, SerRed can be added to hot agarose solution directly and mix well without waiting for the solution to cool. lt is also optional to add SerRed to agarose powder and electrophoresis buffer,which will be heated by microwave to prepare agarose gel. SerRed is compatible with all commonly used electrophoresis buffers.

The precast gel staining protocol is not recommended for polyacrylamide gels. Polyacrylamide gels can be stained using the post-staining protocol.

2.Post-staining

Dilute SerRed 10.000× stock solution 3,300 fold to make a 3×staining solution.(c.g. add 15 ul SerRed 10000× reagent into 50 ml H₂O).

Place the gel carefully into an appropriate container, such as a polypropylene container. Add a sufficient amount of the 3×staining solution slowly to submerge the gel. Shake gently at room temperature for about 30 minutes for staining.The optimal staining time may vary depending on gel thickness and agarose concentration. For 3.5%-10% polyacrylamide gel, the staining time is usually in the range of 30 minutes to 1 hour and extends with the increase of acrylamide concentration.

More dye will be used by post-staining protocol.The 3× SerRed staining solution can be used for about 3 times repeatedly.The 3×SerRed staining solution can be prepared in large quantities and should be stored at room temperature away from light until it is used up.

Note:

1. Cold storage is not required, store at room temperature to avoid precipitation, if precipitation is found, heat SerRed to 45-50 ° C for 2 min with shaking to dissolve without compromising usage.

2. This product stains both ssDNA and RNA, but is less sensitive than dsDNA.

3. Please wear disposable gloves during operation to avoid RNase contamination.

For Research Use Only!