быстрая доставка ~4 недели
Размер
- 1000 U
- 5000 U
- Описание
-
Description
5'…G/CTAGC...3'
3'…CGATC/G...5'Product Components
Components Cat. No. NheI (10, 000 U/ml) RM21613 10X Buffer CutB RM20105 Product Information
Feature Specification Applications Restriction Enzyme Digestion Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 37°C in a total reaction volume of 50 µl. Storage Conditions 10 mM Tris-HCl, 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, 0.15% Triton X-100, pH 7.4 @ 25°C Storage Temperature -20°C Reaction Conditions 1X Buffer CutB, Incubate at 37°C. Reaction Buffer or Mix 1X Buffer CutB:
50 mM NaCl, 10 mM Tris-HC, 10 mM MgCl2, 100 μg/ml BSA, pH 7.9 @ 25°C.Heat Inactivation 65°C for 20 min Methylation Sensitivity dam methylation Not Sensitive dcm methylation Not Sensitive CpG Methylation Blocked by Some Combinations
of OverlappingActivity in Abclonal Buffers CutA CutB CutC CutS 100% 100% 10% 100%