быстрая доставка ~4 недели
Размер
- 1000 U
- 5000 U
StuI (RK21162)
- Описание
-
Description
5'…AGG/CCT...3'
3'…TCC/GGA...5'Product Components
Components Cat. No. StuI (20, 000 U/ml) RM21662 10X Buffer CutU RM20102 Product Information
Feature Specification Applications Restriction Enzyme Digestion Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA fragments in 1 hour at 37°C in a total reaction volume of 50 µl. Storage Conditions 10 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C Storage Temperature -20°C Reaction Conditions 1X Buffer CutU. Incubate at 37°C. Reaction Buffer or Mix 1X Buffer CutU:
20 mM Tris-acetate，10 mM Magnesium acetate，50 mM Potassium acetate，100 µg/ml BSA，pH 7.9 @25℃Heat Inactivation 80°C for 20 min Methylation Sensitivity dam Methylation Not Sensitive dcm Methylation Blocked by Overlapping CpG Methylation Not Sensitive