быстрая доставка ~4 недели
Размер
- 20000 U
- 4000 U
- 50000 U
KpnI (RK21104)
- Описание
-
Description
5'...GGTAC/C...3'
3'...C/CATGG...3'Product Components
Components Cat. No. KpnI (10, 000 U/ml) RM21606 10X Buffer CutA RM20104 Product Information
Feature Specification Applications Restriction Enzyme Digestion Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA fragments in 1 hour at 37°C in a total reaction volume of 50 µl. Storage Conditions 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C Storage Temperature -20°C Reaction Conditions 1X Buffer CutA, Incubate at 37°C. Reaction Buffer or Mix 1X Buffer CutA:
10 mM Bis-Tris-Propane-HCl, 10 mM MgCl2, 100 μg/ml BSA, pH 7 @ 25°CHeat Inactivation No Methylation Sensitivity dam Methylation Not Sensitive dcm Methylation Not Sensitive CpG Methylation Not Sensitive Activity in Abclonal Buffers CutA CutB CutC CutS 100% 75% 10% 50%