быстрая доставка ~4 недели
Размер
- 1250 U
- 250 U
- Описание
-
Description
RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.Product Components
Components Cat. No. RNase H (5, 000 U/ml) RM21305 10X RNase H Reaction Buffer RM20142 Product Information
Feature Specification Product Source An E.coli strain that carries the cloned RNase H gene (rnh) from E. coli. Applications RT-PCR & cDNA Synthesis, RT-qPCR, RT-PCR and cDNA Synthesis, PCR Unit Definition One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 20 picomoles of a fluorescently labelled 50 base pair RNA-DNA hybrid in a total reaction volume of 50 ul in 20 minutes at 37°C. Storage Conditions 10 mM Tris-HCl, 50 mM KCl，0.1 mM EDTA, 1 mM DTT，200 μg/ml BSA，50% Glycerol，pH7.4 @ 25°C Storage Temperature -20°C Reaction Conditions 1X RNase H Reaction Buffer, Incubate at 37°C Reaction Buffer or Mix 1X RNase H Reaction Buffer:
50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25°CHeat Inactivation 65°C for 20 min