быстрая доставка ~4 недели
Размер
- 10 μg
- 100 μg
- 20 μg
- 200 μg
- 5 μg
- 50 μg
- Описание
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Overview
Product name Active Recombinant Human R-spondin-1 Protein Catalog No. RP00071 Description Active Recombinant Human R-spondin-1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg31-Ala263) of human R-Spondin1 (Accession #NP_001033722.1) fused with a 6×His tag at the C-terminus. Bio-Activity Measured by its ability to enhance Cyclin D1 expression in HCT116 human colon adenocarcinoma cells. 0.1-10ng/mL of Recombinant Human RSPO1 can effectively enhance Cyclin D1 expression. Purity > 92% by SDS-PAGE. Endotoxin < 0.1 EU/μg of the protein by LAL method. Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Species Human Background
This protein is a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.Product information
Synonym CRISTIN3; RSPO Expression Host HEK293 cells Tag C-His Swiss-Prot Gene ID Usage guide
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. * For research use only. Not for therapeutic or diagnostic purposes.
Product Protocols