быстрая доставка ~4 недели
Размер
- 10 μg
- 100 μg
- 50 μg
Recombinant Human CD47 Protein (RP02227)
- Описание
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Overview
Product name Recombinant Human CD47 Protein Catalog No. RP02227 Description Recombinant Human CD47 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (CD47(Gln19-Gln141)&CD47(Val198-Ala207)&CD47(Ser257-Gly268)&mFc(Pro99-Lys330)) of human CD47 (Accession #) fused with a Fc and His Tag at the C-terminal. . Bio-Activity ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CD47, mFc-His tagged protein can bind its native ligand Human SIRPα, hFc-His tagged protein in a linear range of 3.3-26.37 ng/mL.|ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CD47, mFc-His tagged protein can bind Anti-CD47 in a linear range of 0.061-1.606 ng/mL. Purity > 95% by SDS-PAGE. Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 15 % trehalose is added as protectants before lyophilization. Species Human Background
Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.Product information
Synonym CD47; MER6; IAP; OA3 Expression Host HEK293 cells Tag C-mFc&His Swiss-Prot Gene ID Usage guide
Storage Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.Reconstitution Reconstitute with deionized water * For research use only. Not for therapeutic or diagnostic purposes.
Product Protocols